wc -l AllValidGSS.txt should be the total of the following three
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wc -l RescueMuXYtuc_memberGSS.txt
grep -v 'XY$' RescueMuEtuc_memberGSS.txt | wc -l
grep '>' RescueMuPlasmidAssembly.fsa | grep '\.' | wc -l