Project

If you know any valuable Mu resources that are not listed here, please let us know. The community involvement is necessary for the success of this resource.

    • Maize Gene Discovery Project: A transposon-tagging strategy by RescueMu was carried out to target maize genes. RescueMu is a derivative of the maize Mu1 transposon and was designed for efficient plasmid rescue in E. coli for reverse genetic analysis. Large quantities of maize mutants, genomic sequences etc were generated by this project and are now available at MaizeGDB.

 

    • Uniform Mu: MuTAIL sequencing strategy to generate large set of seed mutants and to seed mutants.

 

    • Maize Targeted Mutagenesis Database: It is a public repository of maize mutant information including pedigree, phenotype, strain, and DNA sequence. All strains are gridded and barcoded, and can be ordered electronically via this site. It also accepts requests for screening by reverse genetics.

 

    • Maize Endosperm Functional Genomics Consortium: Knockout alleles were created by Mu insertion to identify the genes that control the development of maize endosperm. Large amount of Mu flanking sequences were also generated.

 

    • Mu Killer: Mu killer (Muk) is a single dominant locus reponsible for the epigenetic inactivation of the Mutator transposable element family in maize. Muk results in the heritable transcriptional inactivation of MuDR, the autonomous Mutator family transposon. Maize lines with one or many MuDR elements are silenced by Muk, and silencing is not dependent on the position of MuDR elements.

 

    • IGF consortium: A variety of transposon mutagenesis was produced based on Mu. Mutant plants can be identified by MuArray, MuID, MuAFLP etc techonolgies.

 

  • Photosynthetic Mutant Library (PML): PML is a library of Mu- induced non-photosynthetic maize mutants, corresponding DNA samples, and phenotypic data. The pooled DNA samples can be used as a “reverse genetic resource” to identify mutants with Mu insertions in genes of known sequence with a postulated role in chloroplast biogenesis. The phenotype database can be searched for phenotypes of interest, and corresponding seed requested.